Protocols Protocol: ISO 15985

Protocol: ISO 15985

ISO 15985 (2004)

Anaerobic biodegradation and disintegration under high-solids anaerobic digestion conditions 

  • Anaerobic Biodegradability
  • High-Solids
  • BPC Blue

ISO 15985:2004 is a method for simulating an intensive anaerobic digestion process and determining the ultimate biodegradability and degree of disintegration of a test material under high-solids anaerobic digestion conditions.

Methanogenic inoculum is obtained from anaerobic digesters operating on organic fraction of pretreated household waste. The test material is mixed with inoculum and introduced into a static digestion vessel where it is digested under optimum temperature and moisture conditions for a test period of 15 days or until a plateau in biodegradation has been reached. The biogas production is continuously monitored in test and blank vessels to determine the total biogas production.

Percentage biodegradation is given by the ratio of the amount of biogas evolved from the test material to the maximum theoretical amount of biogas that can be produced from the test material. This percentage biodegradation does not include the amount of carbon converted to new cell biomass which is not metabolised to biogas.

BPC Blue is a fully automated biodegradability testing system suitable for ISO 15985. It allows for continuous and on-line monitoring of released biogas (CH4 and CO2) and is a fully integrated and automated system for sampling, analysing, recording, and generating reports. BPC Blue is the perfect analytical tool that offers standardised measurement procedures, data interpretations and reports.

BPC® Blue

Analytical tool for biodegradability and compostability analysis

Gas Endeavour® III

Precise measurement of gas volume and flow for diverse applications

Application Notes

Experimental conditions

Application notes for utilising ISO 15985 (2004)

Conditions

Non-Mixing
Dark/diffused light (amber bottles available)


Temperature

52 °C


Method & Equipment

Gas volume measurement (biogas)
Gas composition analysis (CH4 / CO2 )


Reactor Size

≥ 0.75 L (1.0 and 2.0 L available with BPC Blue)

Sample

Application notes for utilising ISO 15985 (2004)

Inoculum

From a digester operating under dry conditions (i.e., > 20% TS)

Treated digested sludge from wet fermentation conditions (e.g., dewatered by centrifugation)


Amount of Inoculum

≥ 500 g wet mass


Substrate Form

Powder or fragments up to 2 x 2 cm (all forms supported by BPC Blue)


Substrate Amount

7.5 – 10 g VS


Blank & Controls

Blank: inoculum only
Positive control: TLC graded cellulose < 20 µm


Validation criteria

Application notes for utilising ISO 15985 (2004)

Replicates

3


Test Duration

15 days or until plateau reached.


Other Criteria

Biodegradation of positive control > 70 %

RSD of positive control < 20 %

Related scientific references