Protocols Protocol: ISO 14855-1

Protocol: ISO 14855-1

ISO 14855-1 (2012)

Aerobic biodegradability of plastic materials under controlled composting thermophilic conditions

  • Compostability
  • Plastics
  • BPC Blue

ISO 14855-1:2012 test method determines the ultimate biodegradability and disintegration of test material under conditions simulating an intensive aerobic composting process.

The inoculum used consists of stabilized, mature compost derived, if possible, from composting the organic fraction of solid municipal waste. Test material is mixed with the inoculum and introduced into a composting vessel where it is intensively composted under optimum oxygen, temperature and moisture conditions for a test period not exceeding 6 months. During the aerobic biodegradation of the test material, carbon dioxide, water, mineral salts and new microbial cellular constituents (biomass) are the ultimate biodegradation products.

The evolved CO2 is continuously monitored, or measured at regular intervals, in test and blank vessels to determine the cumulative CO2 production. Percentage biodegradation is given by the ratio between the CO2 produced from the test material and the maximum theoretical amount of CO2 that can be produced from the test material, which is calculated from the measured total organic carbon content.

BPC Blue is a fully automated biodegradability testing system suitable for ISO 14855-1 and may be configured to evaluate aerobic biodegradability of polymer samples under the same temperature range of controlled composting conditions using industrial compost as inoculum. Utilising an in-situ carbon dioxide absorption kit, BPC Blue functions as a closed-respirometer for continuous and on-line measurement of oxygen consumption or depletion caused by aerobic respiration of microorganisms.

BPC® Blue

Analytical tool for biodegradability and compostability analysis

Gas Endeavour® III

Precise measurement of gas volume and flow for diverse applications

Application Notes

Experimental conditions

Application notes for utilising ISO 14855-1 (2012)

Conditions

Non-mixing
Dark/diffused light (amber bottles available)


Temperature

58 °C


Method & Equipment

Gas volume measurement, CO2


Reactor Size

≥ 2.0 L (2.0 L recommended with BPC Blue)

Sample

Application notes for utilising ISO 14855-1 (2012)

Inoculum

Mature compost from municipal solid waste


Inoculum Form

TS = 50 – 55%
pH = 7.0 – 9.0
VSwet > 15%, VSdry > 30%
Particle size 5 – 10 mm
Structural material e.g. wood, vermiculite or perlite (optional)


Amount of Inoculum

200 g TS/L


Substrate Form

Powder or fragments up to 2 x 2 cm (all forms supported by BPC Blue)


Amount of Substrate

33 g TS/L


Inoculum Substrate Ratio ISR

6:1


Blank & Controls

Blank: inoculum only
Positive control e.g. TLC grade cellulose < 20 µm

Validation criteria

Application notes for utilising ISO 14855-1 (2012)

Replicates

3


Test Duration

Until a plateau reached. Maximum 6 months.


Other Criteria

Biodegradation of positive control > 70% after 45 days
RSD of positive control < 20 %
Day 10: Blank > 50 mg < 150 mg CO2 per g VScompost

Related scientific references