Biochemical Methane Potential
Anaerobic batch fermentation assay for the evaluation of biogas and biomethane yield
- Biogas and Biomethane
- Anaerobic Digestion
- AMPTS III
The biomethane potential or biochemical methane potential (BMP) of a specific substrate defines the maximum amount of methane that can be produced by anaerobic digestion. BMP is a key parameter for substrate characterisation and efficiency evaluation of anaerobic digestion plants. It is crucial for assessing quality and monetary value of different substrate types and enables reliable process balancing.
BMP is typically measured in anaerobic batch tests in a laboratory setup (BMP test). The fundamental principle of a BMP test is straight forward: a sample of the investigated substrate is digested with an active and well-functioning inoculum in bottles incubated at an appropriate temperature. Additionally, blank samples, only containing inoculum, are incubated as well. Assuming no synergistic/antagonistic effects, the resulting methane production of the substrate can be determined by subtracting methane production of the blank (inoculum) from the substrate sample (substrate + inoculum). The final value of cumulative methane production after test termination is defined as the experimental BMP of the substrate.
Automatic Methane Potential Test System (AMPTS) III is the research-standard analytical tool preferred by scientists and engineers for conducting various anaerobic batch fermentation tests. AMPTS is the only automated test apparatus referenced in VDI 4630 for biochemical methane potential (BMP), specific methanogenic activity (SMA) and residual gas potential (RGP).
The system consists of 18 parallel reactors (9 reactor configuration available) and the same number of gas flow meters (flow cells) attached to a detection unit for online, automatic data acquisition. This allows for the real-time investigation of a high number of feedstock samples with high precision and accuracy.


AMTPS® III
Analytical tool for biogas research and analysis

BioReactor Simulator® III
Simulate biogas production processes with minimal effort
Application Notes
Experimental conditions
Application notes for conducting BMP tests with AMPTS III
Conditions
Mixing (agitation system included with AMPTS III)
Temperature
Mesophillic (37°C) or thermophillic (55°C)
Method & Equipment
Gas volume measurement (CH4)
Reactor Size
0.5 – 2.0 L
Sample
Application notes for conducting BMP tests with AMPTS III
Inoculum
Digested sludge from municipal sewage waste or agricultural biogas installations.
Amount of Inoculum
400 – 1600 g (depending on reactor size)
Substrate Form
Powder or fragments < 10 mm
Inoculum: Substrate ratio (ISR)
2:1
Blank & Controls
Blank: inoculum only
Positive control e.g. microcrystalline cellulose
Validation criteria
Application notes for conducting BMP tests with AMPTS III
Replicates
3
Test Duration
25-60 days, until a plateau (BMP0.5%)
Other Criteria
Positive control BMP should be within ±10% of the
theoretical (e.g., cellulose: 671-820 biogas or 336-410
CH₄/g VS).
RSD of test material < 15% (homogeneous) or < 20%
heterogeneous)
Related scientific references
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Synergistic valorization of crop residues and dairy wastewater via biochar-mediated anaerobic digestion enhancement
Lan, T., Wen, Y., Tong, L. Ergu, A., Zhang, L., Wu, D., Xing, A., Hong, M.Biomass and Bioenergy (2026) 212
Biomethane, WastewaterJournal article2026 -
Cellulolytic fungi from sewage sludge and their potential to improve methane fermentation
Ćwiertniewicz-Wojciechowska, C., Cema, G.,Ziembińska-Buczyńska, A.Biomass Conversion and Biorefinery (2026) 16:145
BiomethaneJournal article2026 -
Effect of harvesting interval on biogas production from Napier grass
Shah, J., Senapathy, G. J., Bisht, A., Bhagat, M. S., Rasheed, M. A.Energy, Ecology and Environment (2026), 1-15
BiomethaneJournal article2026 -
Reliable biochemical methane potential testing: insights and recommendations from global interlaboratory study
Hafner, S. D., Leca, E., Sambusiti, C., Astals, S., Azam, O., Koch, K., Liu, J., Møller, H. B., Nistor, M., Olaya-Rincon, M., Peyrelasse, C., Ward, A. J., Zennaro, B., Monlau, F. Bioresource Technology (2026) Volume 446
BiomethaneJournal article2026