Protocols Protocol: ASTM D5511-18

Protocol: ASTM D5511-18

ASTM D5511-18

Anaerobic biodegradation of plastic materials under high-solids anaerobic digestion conditions 

  • Anaerobic Biodegradability
  • Plastics
  • High-Solids
  • BPC Blue

ASTM D5511-18 is designed to yield anaerobic biodegradability as percentage of conversion of carbon in the sample to carbon in the gaseous form under conditions found in high-solids anaerobic digesters. The method may also resemble conditions in biologically active landfills where the as generated is recovered and biogas production is actively promoted by inoculation, moisture control and temperature control. Test materials are exposed to a methanogenic inoculum derived from anaerobic digesters operating only on pretreated household waste. The anaerobic decomposition takes place under high-solids (more than 30 % total solids) and non-mixed conditions.

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Application Notes

Experimental conditions

Application notes for utilising ASTM D5511-18

Conditions

Non-Mixing
Dark/diffused light (amber bottles available)


Temperature

37 °C or 52 °C


Method & Equipment

Gas volume measurement (biogas)
Gas composition analysis (CH4 / CO2 )


Reactor Size

2.0 L

Sample

Application notes for utilising ASTM D5511-18

Inoculum

From a digester operating under dry conditions (i.e., > 20% TS)

Treated digested sludge from wet fermentation conditions (e.g., dewatered by centrifugation)


Amount of Inoculum

1000 g wet mass


Substrate Form

Powder or fragments up to 10 x 5 x 0.4 cm


Substrate Amount

15 – 100 g VS


Blank & Controls

Blank: inoculum only
Positive control: TLC graded cellulose < 20 µm
Negative control: PE (optional)


Validation criteria

Application notes for utilising ASTM D5511-18

Replicates

3


Test Duration

30 days or until plateau reached.


Other Criteria

Biodegradation of positive control > 70 %

RSD of positive control < 20 %

Related scientific references